Kužela seminar series
Fri 20 May. 2011, 13:00
Title: RNA editing and mitochondrial translation in trypanosomes
Speaker: Dmitri Maslov, University of California Riverside, USA
U-insertion/deletion RNA editing in trypanosomes creates translatable reading frames from translation-incompetent pre-edited mRNA. The process of editing is mediated by small guide RNAs that perform their role in a highly ordered fashion beginning at the 3' end of a pre-edited transcript and proceeding in the 5' direction. The process of editing is coupled with addition of long poly(A/U) tails which signify translation- competence of the mRNA. Translation is performed by 50S monosomes which display a unique combination of conserved and derived features. It is hypothesized that in order to recognize the mRNA, the monosomes interact with other protein complexes, particularly those which contain pentatricopeptide repeat (PPR) proteins.