Bioinformatický seminár

Tue 20 Dec. 2011, 17:20

Title: Roy et al. Identification of functional elements and regulatory circuits by Drosophila modENCODE
Speaker: Lukáš Bača, Andrej Kiss

To gain insight into how genomic information is translated into cellular
and developmental programs, the Drosophila model organism Encyclopedia of
DNA Elements (modENCODE) project is comprehensively mapping transcripts,
histone modifications, chromosomal proteins, transcription factors,
replication proteins and intermediates, and nucleosome properties across a
developmental time course and in multiple cell lines. We have generated
more than 700 data sets and discovered protein-coding, noncoding, RNA
regulatory, replication, and chromatin elements, more than tripling the
annotated portion of the Drosophila genome. Correlated activity patterns
of these elements reveal a functional regulatory network, which predicts
putative new functions for genes, reveals stage- and tissue-specific
regulators, and enables gene-expression prediction. Our results provide a
foundation for directed experimental and computational studies in
Drosophila and related species and also a model for systematic data
integration toward comprehensive genomic and functional annotation.