Bioinformatický seminár

Tue 13 Nov. 2012, 17:20

Title: Elizabeth P. Murchison et al. (2012) Genome sequencing and analysis of the Tasmanian devil and its transmissiblecancer
Speaker: Iveta Halanova

The Tasmanian devil (Sarcophilus harrisii), the largest marsupial carnivore, is
endangered due to a transmissible facial cancer spread by direct transfer of
living cancer cells through biting. Here we describe the sequencing, assembly,
and annotation of the Tasmanian devil genome and whole-genome sequences for two
geographically distant subclones of the cancer. Genomic analysis suggests that
the cancer first arose from a female Tasmanian devil and that the clone has
subsequently genetically diverged during its spread across Tasmania. The devil
cancer genome contains more than 17,000 somatic base substitution mutations and
bears the imprint of a distinct mutational process. Genotyping of somatic
mutations in 104 geographically and temporally distributed Tasmanian devil tumors
reveals the pattern of evolution and spread of this parasitic clonal lineage,
with evidence of a selective sweep in one geographical area and persistence of
parallel lineages in other populations.