CB-aln-dp: Rozdiel medzi revíziami

Aktuálna revízia z 16:24, 14. október 2021

Sekvencie a zarovnania k cvičeniu pre biológov: programy water a needle

Obsah

1 Proteínové sekvencie
2 Lokálne zarovnanie
3 Globálne zarovnanie
4 Globálne zarovnanie s nulovou penaltou medzier na koncoch sekvencie

Proteínové sekvencie

>sp|P50520|VPS34_SCHPO Phosphatidylinositol 3-kinase vps34 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vps34 PE=2 SV=2
MDRLVFSYCPSSKVTARFLVKFCFIEYQDSQEPCICTIQLFSGNESGSLMQKCFVSKIPN
KSLLPTELSKISTHEWLDFGVTVSELSLNAKFVVSAWKPSFNDEEVYEFVGCTTYRLFDE
NNLLRQGLQKIPLQTSKEIKKYSPTSLELEQVKEINRLDGLLLKLQLGDVPSVNWLDDIS
FGKIKDFRSKHMSLVTIPILYLDFLQFSFPVVFQRSYYPKSENRVYYSSFDLELNLDSPA
ELKHRRLVRSQRNGPLDKDLKPNSKIRKELESILSYPPSEELSLEEKDLIWKFRFYLTRN
KKAMTKFLKSVVWTDSSEVNQALSLLDSWTEIDIDDALELLSPSFVHPKVRAYAVSRLET
ASNEELLLYLLQLVQALRYDNPISSDERFQPSPLALFLVNRAISSPSIGNDLYWYLVVEI
EDEPVSKLFSSVMFLFQKELSKSVEGRLIRETLSAQAKFVEKLLRISKSVQSFRGTRLKK
IEYLKVLLEDHKYHLLDFHALPLPLDPSVNIVGIIPDACTVFKSTMQPLRLLFKCQDGSK
YPIIFKNGDDLRQDQLVIQILTLMDKLLKKEKLDLHLKPYRILATGPTHGAVQFVPSKTL
ATILAEYHGSVLAYLRENNPDDGLNSANYGIDPVAMDNYVRSCAGYCVITYLLGVGDRHL
DNLLITKDGHFFHADFGYILGRDPKLFSPAMKLSKEMVEGMGGYNSPFYQQFKSYCYTTF
TALRKSSNLILNLFSLMVDANIPDIKFDKEKVVYKVKERFCLQMSESDAIKYFEQLINDS
VSALFPQIIDRMHNLAQYMRS

>RKM3_YEAST
MSVTFKDDVHRILKFVANCNGRFEDSKCDIRESPLGGLGVFAKTDIAEGESILTLNKSSI
FSASNSSIANLLCDSSIDGMLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPP
SFWHADAKRLLKGTSFDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSE
ENHEEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLYDV
CDKCGEPDMCKHLIAEEYLEAENLDKNMPKVASMETRVIDEDLIKSLENDLEKEYSNVTA
NIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLARYGFTVPENQYDIVHL
GPDFMKILKKEEKYQEKVKWWSQVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESE
EEEEEEEGDDSLESWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESLFSKKATPHISDS
IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQNARTLVQSE
HNILDRCLKRLS

Lokálne zarovnanie

########################################
# Program: water
# Rundate: Thu 14 Oct 2021 15:08:56
# Commandline: water
#    -auto
#    -stdout
#    -asequence emboss_water-I20211014-150851-0938-20653107-p2m.asequence
#    -bsequence emboss_water-I20211014-150851-0938-20653107-p2m.bsequence
#    -datafile EBLOSUM62
#    -gapopen 10.0
#    -gapextend 0.5
#    -aformat3 pair
#    -sprotein1
#    -sprotein2
# Align_format: pair
# Report_file: stdout
########################################

#=======================================
#
# Aligned_sequences: 2
# 1: MCA_00027_1
# 2: RKM3_YEAST
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 588
# Identity:     170/588 (28.9%)
# Similarity:   270/588 (45.9%)
# Gaps:         116/588 (19.7%)
# Score: 611.0
# 
#
#=======================================

MCA_00027_1       29 IRESPLGGIGVFARKKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDG     78
                     ||||||||:||||:..|.:...||.:.|:.|.|.||.||:|||.::..||
RKM3_YEAST        30 IRESPLGGLGVFAKTDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDG     79

MCA_00027_1       79 MLGLALAYMYERSLGPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEE    124
                     ||.|.:|::||.::..:|. ||.|::||   |.:..::..|.||..:.:.
RKM3_YEAST        80 MLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKR    129

MCA_00027_1      125 LLVGTELYYHTL---KVEDDDIAEVFKFDV------------------LP    153
                     ||.||.  :.||   ...:::|.|.|:..|                  |.
RKM3_YEAST       130 LLKGTS--FDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLD    177

MCA_00027_1      154 FLERNHLFEGQPQYR-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLF    202
                     ..|.||    :..|. .||.:......::||.|::|.||..||||.|.||
RKM3_YEAST       178 VSEENH----EEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLF    223

MCA_00027_1      203 NHSI-DESVHFEVVSQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVD    251
                     ||.: |..:.|..:..||..||.||.|.||.        ::|..|.|.:|
RKM3_YEAST       224 NHHVSDPDLKFVSLYDVCDKCGEPDMCKHLI--------AEEYLEAENLD    265

MCA_00027_1      252 ELNEDGFSSGFDYSDSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSD    301
                     :....                              :.|.||.: |.||..
RKM3_YEAST       266 KNMPK------------------------------VASMETRV-IDEDLI    284

MCA_00027_1      302 KKTSNEQKSEEQEAEKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEV    351
                     |...|:.:.|.....    ...::|:...:.|.:..|:|....|.:|.|:
RKM3_YEAST       285 KSLENDLEKEYSNVT----ANIEDDDGGIENPDECVDLVLKNDVAQGQEI    330

MCA_00027_1      352 FNTYGELSNHHLASRYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEW    400
                     ||:||||||..|.:||||.:.:|:|:.|.|.|:..:.: .|....|:.:|
RKM3_YEAST       331 FNSYGELSNVFLLARYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKW    380

MCA_00027_1      401 WSIYFYEALFGIRKDEWAEIEESEDEDDEG------------SEDSEEEN    438
                     ||    :...|:....:|::.:.::||::|            ||:.|||.
RKM3_YEAST       381 WS----QVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEE    426

MCA_00027_1      439 SIPPSPESISWEDEAYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFER    488
                     ....|.|  ||..:.|:.:||.||.....|..:|:::...:|:|      
RKM3_YEAST       427 EGDDSLE--SWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESL------    468

MCA_00027_1      489 DVFTSKLLPS-STLVFNEKSLILLKALVNLRLQRYKDGMLTSSQI-----    532
                       |:.|..|. |..:.||:.|..|....|...::....:|...|:     
RKM3_YEAST       469 --FSKKATPHISDSIVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKG    516

MCA_00027_1      533 --KDLITKLSDNQKKNSRQILALTIKGTEKIVLEKSMQ    568
                       ...||..:.:..:|:|     |:..:|..:|::.::
RKM3_YEAST       517 DNSSKITSATKSMLQNAR-----TLVQSEHNILDRCLK    549

Globálne zarovnanie

########################################
# Program: needle
# Rundate: Thu 14 Oct 2021 15:13:34
# Commandline: needle
#    -auto
#    -stdout
#    -asequence emboss_needle-I20211014-151332-0965-44565643-p2m.asequence
#    -bsequence emboss_needle-I20211014-151332-0965-44565643-p2m.bsequence
#    -datafile EBLOSUM62
#    -gapopen 10.0
#    -gapextend 0.5
#    -endweight
#    -endopen 10.0
#    -endextend 0.5
#    -aformat3 pair
#    -sprotein1
#    -sprotein2
# Align_format: pair
# Report_file: stdout
########################################

#=======================================
#
# Aligned_sequences: 2
# 1: MCA_00027_1
# 2: RKM3_YEAST
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 650
# Identity:     178/650 (27.4%)
# Similarity:   282/650 (43.4%)
# Gaps:         153/650 (23.5%)
# Score: 588.5
# 
#
#=======================================

MCA_00027_1        1 MEDSL---MERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFARK     43
                     |..:.   :.|...||    |...|:|..      ||||||||:||||:.
RKM3_YEAST         1 MSVTFKDDVHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAKT     44

MCA_00027_1       44 KIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSLG     93
                     .|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.::.
RKM3_YEAST        45 DIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTVF     94

MCA_00027_1       94 PDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL---    136
                     .:|. ||.|::||   |.:..::..|.||..:.:.||.||.  :.||   
RKM3_YEAST        95 RNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFDS    142

MCA_00027_1      137 KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQYR    168
                     ...:::|.|.|:..|                  |...|.||    :..|.
RKM3_YEAST       143 LAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDYN    188

MCA_00027_1      169 -TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVVS    216
                      .||.:......::||.|::|.||..||||.|.||||.: |..:.|..:.
RKM3_YEAST       189 LKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLY    238

MCA_00027_1      217 QVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYSD    266
                     .||..||.||.|.||.        ::|..|.|.:|:....          
RKM3_YEAST       239 DVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK----------    270

MCA_00027_1      267 SESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEAE    316
                                         :.|.||.: |.||..|...|:.:.|.....
RKM3_YEAST       271 --------------------VASMETRV-IDEDLIKSLENDLEKEYSNVT    299

MCA_00027_1      317 KYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLASR    366
                         ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.:|
RKM3_YEAST       300 ----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLAR    345

MCA_00027_1      367 YGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRKD    415
                     |||.:.:|:|:.|.|.|:..:.: .|....|:.:|||    :...|:...
RKM3_YEAST       346 YGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFSA    391

MCA_00027_1      416 EWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDEA    453
                     .:|::.:.::||::|            ||:.|||.....|.|  ||..:.
RKM3_YEAST       392 WYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQL    439

MCA_00027_1      454 YLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STLV    502
                     |:.:||.||.....|..:|:::...:|:|        |:.|..|. |..:
RKM3_YEAST       440 YIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDSI    481

MCA_00027_1      503 FNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQKK    545
                     .||:.|..|....|...::....:|...|:       ...||..:.:..:
RKM3_YEAST       482 VNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQ    531

MCA_00027_1      546 NSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKLK    595
                     |:|     |:..:|..:|::.:                        ::|.
RKM3_YEAST       532 NAR-----TLVQSEHNILDRCL------------------------KRLS    552

Globálne zarovnanie s nulovou penaltou medzier na koncoch sekvencie

########################################
# Program: needle
# Rundate: Thu 14 Oct 2021 15:05:37
# Commandline: needle
#    -auto
#    -stdout
#    -asequence emboss_needle-I20211014-151156-0154-17922699-p1m.asequence
#    -bsequence emboss_needle-I20211014-151156-0154-17922699-p1m.bsequence
#    -datafile EBLOSUM62
#    -gapopen 10.0
#    -gapextend 0.5
#    -endopen 10.0
#    -endextend 0.5
#    -aformat3 pair
#    -sprotein1
#    -sprotein2
# Align_format: pair
# Report_file: stdout
########################################

#=======================================
#
# Aligned_sequences: 2
# 1: MCA_00027_1
# 2: RKM3_YEAST
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 651
# Identity:     177/651 (27.2%)
# Similarity:   282/651 (43.3%)
# Gaps:         155/651 (23.8%)
# Score: 608.0
# 
#
#=======================================

MCA_00027_1        1 ----MEDSLMERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFAR     42
                         .:|. :.|...||    |...|:|..      ||||||||:||||:
RKM3_YEAST         1 MSVTFKDD-VHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAK     43

MCA_00027_1       43 KKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSL     92
                     ..|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.::
RKM3_YEAST        44 TDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTV     93

MCA_00027_1       93 GPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL--    136
                     ..:|. ||.|::||   |.:..::..|.||..:.:.||.||.  :.||  
RKM3_YEAST        94 FRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFD    141

MCA_00027_1      137 -KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQY    167
                      ...:::|.|.|:..|                  |...|.||    :..|
RKM3_YEAST       142 SLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDY    187

MCA_00027_1      168 R-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVV    215
                     . .||.:......::||.|::|.||..||||.|.||||.: |..:.|..:
RKM3_YEAST       188 NLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSL    237

MCA_00027_1      216 SQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYS    265
                     ..||..||.||.|.||.        ::|..|.|.:|:....         
RKM3_YEAST       238 YDVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK---------    270

MCA_00027_1      266 DSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEA    315
                                          :.|.||.: |.||..|...|:.:.|....
RKM3_YEAST       271 ---------------------VASMETRV-IDEDLIKSLENDLEKEYSNV    298

MCA_00027_1      316 EKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLAS    365
                     .    ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.:
RKM3_YEAST       299 T----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLA    344

MCA_00027_1      366 RYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRK    414
                     ||||.:.:|:|:.|.|.|:..:.: .|....|:.:|||    :...|:..
RKM3_YEAST       345 RYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFS    390

MCA_00027_1      415 DEWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDE    452
                     ..:|::.:.::||::|            ||:.|||.....|.|  ||..:
RKM3_YEAST       391 AWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQ    438

MCA_00027_1      453 AYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STL    501
                     .|:.:||.||.....|..:|:::...:|:|        |:.|..|. |..
RKM3_YEAST       439 LYIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDS    480

MCA_00027_1      502 VFNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQK    544
                     :.||:.|..|....|...::....:|...|:       ...||..:.:..
RKM3_YEAST       481 IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSML    530

MCA_00027_1      545 KNSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKL    594
                     :|:|     |:..:|..:|::.::.::                       
RKM3_YEAST       531 QNAR-----TLVQSEHNILDRCLKRLS-----------------------    552

MCA_00027_1      595 K    595
                      
RKM3_YEAST       553 -    552

CB-aln-dp: Rozdiel medzi revíziami

Aktuálna revízia z 16:24, 14. október 2021

Obsah

Proteínové sekvencie

Lokálne zarovnanie

Globálne zarovnanie

Globálne zarovnanie s nulovou penaltou medzier na koncoch sekvencie

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@@ Riadok 2: / Riadok 2: @@
 ==Proteínové sekvencie==
+* http://genome.compbio.fmph.uniba.sk/data/magCapA5/proteins/MCA_00027_1.html
+* https://www.uniprot.org/uniprot/P38222#sequences
 <pre>
 >sp|P50520|VPS34_SCHPO Phosphatidylinositol 3-kinase vps34 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vps34 PE=2 SV=2
@@ Riadok 21: / Riadok 23: @@
 <pre>
->tr|B1AKP8|B1AKP8_HUMAN FK506 binding protein 12-rapamycin associated protein 1 OS=Homo sapiens GN=FRAP1 PE=4 SV=1
+>RKM3_YEAST
-MNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAES
+MSVTFKDDVHRILKFVANCNGRFEDSKCDIRESPLGGLGVFAKTDIAEGESILTLNKSSI
-TENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGH
+FSASNSSIANLLCDSSIDGMLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPP
-WPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYP
+SFWHADAKRLLKGTSFDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSE
-LTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEAS
+ENHEEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLYDV
-RLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVK
+CDKCGEPDMCKHLIAEEYLEAENLDKNMPKVASMETRVIDEDLIKSLENDLEKEYSNVTA
-DLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQS
+NIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLARYGFTVPENQYDIVHL
-IAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTS
+GPDFMKILKKEEKYQEKVKWWSQVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESE
-LRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDY
+EEEEEEEGDDSLESWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESLFSKKATPHISDS
-DHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILG
+IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQNARTLVQSE
-LGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGN
+HNILDRCLKRLS
-YRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSV
-EILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSH
-DDTLDVPTQVELLIKQATSHENLCQCYIGWCPFW
 </pre>
@@ Riadok 41: / Riadok 40: @@
 ########################################
 # Program: water
-# Rundate: Thu 11 Oct 2012 09:37:58
+# Rundate: Thu 14 Oct 2021 15:08:56
 # Commandline: water
-#    -asequence ggg1
+#    -auto
-#    -bsequence ggg2
+#    -stdout
-#    -outfile ggg12.w
+#    -asequence emboss_water-I20211014-150851-0938-20653107-p2m.asequence
-# Align_format: srspair
+#    -bsequence emboss_water-I20211014-150851-0938-20653107-p2m.bsequence
-# Report_file: ggg12.w
+#    -datafile EBLOSUM62
+#    -gapopen 10.0
+#    -gapextend 0.5
+#    -aformat3 pair
+#    -sprotein1
+#    -sprotein2
+# Align_format: pair
+# Report_file: stdout
 ########################################
@@ Riadok 53: / Riadok 59: @@
 #
 # Aligned_sequences: 2
-# 1: VPS34_SCHPO
+# 1: MCA_00027_1
-# 2: B1AKP8_HUMAN
+# 2: RKM3_YEAST
 # Matrix: EBLOSUM62
 # Gap_penalty: 10.0
 # Extend_penalty: 0.5
 #
-# Length: 645
+# Length: 588
-# Identity:     124/645 (19.2%)
+# Identity:     170/588 (28.9%)
-# Similarity:   221/645 (34.3%)
+# Similarity:   270/588 (45.9%)
-# Gaps:         211/645 (32.7%)
+# Gaps:         116/588 (19.7%)
-# Score: 226.0
+# Score: 611.0
 #
 #
 #=======================================
-VPS34_SCHPO      235 NLDSPAELKHRRLVRSQRNGPLDKDLKPNSKIRKELESILSYPPSEELSL    284
+MCA_00027_1       29 IRESPLGGIGVFARKKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDG     78
-                      |.:|.||        |..|.|....|:  .|:.::|...|
+                      ||||||||:||||:..|.:...||.:.|:.|.|.||.||:|||.::..||
-B1AKP8_HUMAN      53 NSESEAE--------STENSPTPSPLQ--KKVTEDLSKTL----------     82
+RKM3_YEAST        30 IRESPLGGLGVFAKTDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDG     79
-VPS34_SCHPO      285 EEKDLIWKFRFYLTRNKKAMTKFLKSVVWTDSSEVNQALSLLDSWTEI--    332
+MCA_00027_1       79 MLGLALAYMYERSLGPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEE    124
-                                 |.....|:..|.:|:..:..:.:...|.:|..|.:.
+                     ||.|.:|::||.::..:|. ||.|::||   |.:..::..|.||..:.:.
-B1AKP8_HUMAN      83 ------------LMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGH    120
+RKM3_YEAST        80 MLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKR    129
-VPS34_SCHPO      333 --DIDDALELLSPSFVHPKVRAYAVSRLETASNEELLLYLLQLVQAL--R    378
+MCA_00027_1      125 LLVGTELYYHTL---KVEDDDIAEVFKFDV------------------LP    153
-                       |:::||           |......:::|         .||::..|  |
+                     ||.||.  :.||   ...:::|.|.|:..|                  |.
-B1AKP8_HUMAN     121 WPDVNEAL-----------VEGVKAIQIDT---------WLQVIPQLIAR    150
+RKM3_YEAST       130 LLKGTS--FDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLD    177
-VPS34_SCHPO      379 YDNP-----------ISSDERFQPS----PLAL-----------------    396
+MCA_00027_1      154 FLERNHLFEGQPQYR-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLF    202
-                      .|.|           ::...|:.|.    ||.:
+                      ..|.||    :..|. .||.:......::||.|::|.||..||||.|.||
-B1AKP8_HUMAN     151 IDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKIL    200
+RKM3_YEAST       178 VSEENH----EEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLF    223
-VPS34_SCHPO      397 --------FLVNRA--ISSPSIGNDLYWYLVVEIEDEPVSKLF---SSVM    433
+MCA_00027_1      203 NHSI-DESVHFEVVSQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVD    251
-                             .||.:|  :|...|...:.|:.:.....|..|:|:   .:|.
+                     ||.: |..:.|..:..||..||.||.|.||.        ::|..|.|.:|
-B1AKP8_HUMAN     201 KNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVK    250
+RKM3_YEAST       224 NHHVSDPDLKFVSLYDVCDKCGEPDMCKHLI--------AEEYLEAENLD    265
-VPS34_SCHPO      434 FLFQ----------------KELS-----------------KSVEGRLIR    450
+MCA_00027_1      252 ELNEDGFSSGFDYSDSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSD    301
-                     .:|:                ||.|                 |.::...::
+                     :....                              :.|.||.: |.||..
-B1AKP8_HUMAN     251 GMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVK    300
+RKM3_YEAST       266 KNMPK------------------------------VASMETRV-IDEDLI    284
-VPS34_SCHPO      451 ETLSAQAKFVEKLLRISKSVQSFRGTRLKKIEYLKVLLEDHKYHLLDFHA    500
+MCA_00027_1      302 KKTSNEQKSEEQEAEKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEV    351
-                      :...|...:.....||||.:.......|:.:....::..|.:.      |
+                      |...|:.:.|.....    ...::|:...:.|.:..|:|....|.:|.|:
-B1AKP8_HUMAN     301 DLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLEL------A    344
+RKM3_YEAST       285 KSLENDLEKEYSNVT----ANIEDDDGGIENPDECVDLVLKNDVAQGQEI    330
-VPS34_SCHPO      501 LPLPLDPSVNIVGI--IPDACTVFKSTMQPLRLLFKCQDGSKYPIIFKNG    548
+MCA_00027_1      352 FNTYGELSNHHLASRYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEW    400
-                      :|...||:..|:.|  |..:..|..|..:|.:|.....:|.::..:.|..
+                      ||:||||||..|.:||||.:.:|:|:.|.|.|:..:.: .|....|:.:|
-B1AKP8_HUMAN     345 VPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGH    394
+RKM3_YEAST       331 FNSYGELSNVFLLARYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKW    380
-VPS34_SCHPO      549 DDLRQDQLVIQILTLMDKLLKKE----KLDLHLKPYRILATGPTHGAVQF    594
+MCA_00027_1      401 WSIYFYEALFGIRKDEWAEIEESEDEDDEG------------SEDSEEEN    438
-                      :|||||:.|:|:..|::.||..:    :.:|.::.|.::......|.:.:
+                      ||    :...|:....:|::.:.::||::|            ||:.|||.
-B1AKP8_HUMAN     395 EDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGW    444
+RKM3_YEAST       381 WS----QVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEE    426
-VPS34_SCHPO      595 VP-SKTLATILAEYHG-----------------------------SVLAY    614
+MCA_00027_1      439 SIPPSPESISWEDEAYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFER    488
-                      || ..||..::.:|..                             .|..:
+                      ....|.|  ||..:.|:.:||.||.....|..:|:::...:|:|
-B1AKP8_HUMAN     445 VPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEH    494
+RKM3_YEAST       427 EGDDSLE--SWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESL------    468
-VPS34_SCHPO      615 LRENNPDDGLNSANYGIDP---VAMD---NYVRSCAGYCVITYLLGVGDR    658
+MCA_00027_1      489 DVFTSKLLPS-STLVFNEKSLILLKALVNLRLQRYKDGMLTSSQI-----    532
-                     ...|...|.|....:...|   |..|   ||.||.|...::.|:||:|||
+                       |:.|..|. |..:.||:.|..|....|...::....:|...|:
-B1AKP8_HUMAN     495 AVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDR    544
+RKM3_YEAST       469 --FSKKATPHISDSIVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKG    516
-VPS34_SCHPO      659 HLDNLLITK-DGHFFHADFG--------------YILGRDPKLFSPAMKL    693
+MCA_00027_1      533 --KDLITKLSDNQKKNSRQILALTIKGTEKIVLEKSMQ    568
-                     |..||::.: .|...|.|||              .|..|..::.:.||:
+                       ...||..:.:..:|:|     |:..:|..:|::.::
-B1AKP8_HUMAN     545 HPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAME-    593
+RKM3_YEAST       517 DNSSKITSATKSMLQNAR-----TLVQSEHNILDRCLK    549
-VPS34_SCHPO      694 SKEMVEGMGGYNSPFYQQFKSYCYTTFTALRKSSNLILNLFSLMV    738
-                         |.|:.|       .::..|:|....||:..:.::.:....|
-B1AKP8_HUMAN     594 ----VTGLDG-------NYRITCHTVMEVLREHKDSVMAVLEAFV    627
 </pre>
@@ Riadok 125: / Riadok 127: @@
 ########################################
 # Program: needle
-# Rundate: Thu 11 Oct 2012 09:40:40
+# Rundate: Thu 14 Oct 2021 15:13:34
 # Commandline: needle
-#    -asequence ggg1
+#    -auto
-#    -bsequence ggg2
+#    -stdout
+#    -asequence emboss_needle-I20211014-151332-0965-44565643-p2m.asequence
+#    -bsequence emboss_needle-I20211014-151332-0965-44565643-p2m.bsequence
+#    -datafile EBLOSUM62
+#    -gapopen 10.0
+#    -gapextend 0.5
 #    -endweight
-#    -outfile ggg12.n2
+#    -endopen 10.0
-# Align_format: srspair
+#    -endextend 0.5
-# Report_file: ggg12.n2
+#    -aformat3 pair
+#    -sprotein1
+#    -sprotein2
+# Align_format: pair
+# Report_file: stdout
 ########################################
@@ Riadok 138: / Riadok 149: @@
 #
 # Aligned_sequences: 2
-# 1: VPS34_SCHPO
+# 1: MCA_00027_1
-# 2: B1AKP8_HUMAN
+# 2: RKM3_YEAST
 # Matrix: EBLOSUM62
 # Gap_penalty: 10.0
 # Extend_penalty: 0.5
 #
-# Length: 948
+# Length: 650
-# Identity:     167/948 (17.6%)
+# Identity:     178/650 (27.4%)
-# Similarity:   292/948 (30.8%)
+# Similarity:   282/650 (43.4%)
-# Gaps:         341/948 (36.0%)
+# Gaps:         153/650 (23.5%)
-# Score: 130.5
+# Score: 588.5
 #
 #
 #=======================================
-VPS34_SCHPO        1 MD-RLVFSYCPSSKVTARFLVKFCFIEYQDSQEPCICTIQLFSGNESGSL     49
+MCA_00027_1        1 MEDSL---MERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFARK     43
-                      |: ..|..|                 ::|:            ...:....
+                      |..:.   :.|...||    |...|:|..      ||||||||:||||:.
-B1AKP8_HUMAN       1 MNFEAVLHY-----------------KHQN------------QARDEKKK     21
+RKM3_YEAST         1 MSVTFKDDVHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAKT     44
-VPS34_SCHPO       50 MQKCFVSKIPNKSLLPTELSKISTHEWLDFGVTVSELSLNAKFVVSAWKP     99
+MCA_00027_1       44 KIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSLG     93
-                      ::....:.|.|.:...|..:..:|                     :|...
+                      .|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.::.
-B1AKP8_HUMAN      22 LRHASGANITNATTAATTAATATT---------------------TASTE     50
+RKM3_YEAST        45 DIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTVF     94
-VPS34_SCHPO      100 SFNDEEVYEFVGCTTYRLFDENNLLRQGLQ-KIPLQTSKEIKKYSPTSLE    148
+MCA_00027_1       94 PDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL---    136
-                      ..|.|...|..         ||:.....|| |:....||.:..|:..:::
+                      .:|. ||.|::||   |.:..::..|.||..:.:.||.||.  :.||
-B1AKP8_HUMAN      51 GSNSESEAEST---------ENSPTPSPLQKKVTEDLSKTLLMYTVPAVQ     91
+RKM3_YEAST        95 RNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFDS    142
-VPS34_SCHPO      149 ----LEQVKEINRLDGLLLKLQLGDVPSVNWLDDISFGKIKDFRSKHMSL    194
+MCA_00027_1      137 KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQYR    168
-                         ...:...|.|...|..|.|       |.|   :|...|...   :|
+                     ...:::|.|.|:..|                  |...|.||    :..|.
-B1AKP8_HUMAN      92 GFFRSISLSRGNNLQDTLRVLTL-------WFD---YGHWPDVNE---AL    128
+RKM3_YEAST       143 LAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDYN    188
-VPS34_SCHPO      195 VT-IPILYLDFLQFSFPVVFQRSYYPKSENRVYYSSFDLELNLDSP----    239
+MCA_00027_1      169 -TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVVS    216
-                     |. :..:.:|......|.:..|                    :|:|
+                      .||.:......::||.|::|.||..||||.|.||||.: |..:.|..:.
-B1AKP8_HUMAN     129 VEGVKAIQIDTWLQVIPQLIAR--------------------IDTPRPLV    158
+RKM3_YEAST       189 LKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLY    238
-VPS34_SCHPO      240 AELKHRRLVRSQRNGPLDKDLKPNSKIRKELESILSYPPSEELSLEEKDL    289
+MCA_00027_1      217 QVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYSD    266
-                      ..|.|:.|....|..|                ..|.||    |::..|..
+                      .||..||.||.|.||.        ::|..|.|.:|:....
-B1AKP8_HUMAN     159 GRLIHQLLTDIGRYHP----------------QALIYP----LTVASKST    188
+RKM3_YEAST       239 DVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK----------    270
-VPS34_SCHPO      290 IWKFRFYLTRNKKAMTKFLKSVVWTDSSEVNQALSLLDSWTEIDI-----    334
+MCA_00027_1      267 SESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEAE    316
-                            .|....|..|.||::....::.|.||:.:.:....:.|
+                                         :.|.||.: |.||..|...|:.:.|.....
-B1AKP8_HUMAN     189 -------TTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEM    231
+RKM3_YEAST       271 --------------------VASMETRV-IDEDLIKSLENDLEKEYSNVT    299
-VPS34_SCHPO      335 -DDALELLSPSFVHPKVRAYAVSRLETASNEELLLYLLQLVQALRYDNPI    383
+MCA_00027_1      317 KYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLASR    366
-                      .:.||..|        |.|...|     |.:.:..:|:.:.|:.
+                         ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.:|
-B1AKP8_HUMAN     232 WHEGLEEAS--------RLYFGER-----NVKGMFEVLEPLHAMM-----    263
+RKM3_YEAST       300 ----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLAR    345
-VPS34_SCHPO      384 SSDERFQPSPLALFLVNRAISSPSIGNDLYWYLVVEIEDEPVSKLFSSVM    433
+MCA_00027_1      367 YGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRKD    415
-                        || .|..|.....|:|     .|.||     :|.
+                     |||.:.:|:|:.|.|.|:..:.: .|....|:.:|||    :...|:...
-B1AKP8_HUMAN     264 ---ER-GPQTLKETSFNQA-----YGRDL-----MEA-------------    286
+RKM3_YEAST       346 YGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFSA    391
-VPS34_SCHPO      434 FLFQKELSKSVEGRLIRETLSAQAKFVEKLLRISKSVQSFRGTRLKKIEY    483
+MCA_00027_1      416 EWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDEA    453
-                        |:...|.::...:::...|...:.....||||.:.......|:.:..
+                     .:|::.:.::||::|            ||:.|||.....|.|  ||..:.
-B1AKP8_HUMAN     287 ---QEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSP    333
+RKM3_YEAST       392 WYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQL    439
-VPS34_SCHPO      484 LKVLLEDHKYHLLDFHALPLPLDPSVNIVGI--IPDACTVFKSTMQPLRL    531
+MCA_00027_1      454 YLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STLV    502
-                      ..::..|.:.      |:|...||:..|:.|  |..:..|..|..:|.:|
+                      |:.:||.||.....|..:|:::...:|:|        |:.|..|. |..:
-B1AKP8_HUMAN     334 KLLMCRDLEL------AVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKL    377
+RKM3_YEAST       440 YIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDSI    481
-VPS34_SCHPO      532 LFKCQDGSKYPIIFKNGDDLRQDQLVIQILTLMDKLLKKE----KLDLHL    577
+MCA_00027_1      503 FNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQKK    545
-                      .....:|.::..:.|..:|||||:.|:|:..|::.||..:    :.:|.:
+                      .||:.|..|....|...::....:|...|:       ...||..:.:..:
-B1AKP8_HUMAN     378 TLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSI    427
+RKM3_YEAST       482 VNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQ    531
-VPS34_SCHPO      578 KPYRILATGPTHGAVQFVP-SKTLATILAEYHG-----------------    609
+MCA_00027_1      546 NSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKLK    595
-                      :.|.::......|.:.:|| ..||..::.:|..
+                      |:|     |:..:|..:|::.:                        ::|.
-B1AKP8_HUMAN     428 QRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMA    477
+RKM3_YEAST       532 NAR-----TLVQSEHNILDRCL------------------------KRLS    552
-VPS34_SCHPO      610 ------------SVLAYLRENNPDDGLNSANYGIDP---VAMD---NYVR    641
-                                 .|..:...|...|.|....:...|   |..|   ||.|
-B1AKP8_HUMAN     478 PDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTR    527
-VPS34_SCHPO      642 SCAGYCVITYLLGVGDRHLDNLLITK-DGHFFHADFG-------------    677
-                     |.|...::.|:||:||||..||::.: .|...|.|||
-B1AKP8_HUMAN     528 SLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFP    577
-VPS34_SCHPO      678 -YILGRDPKLFSPAMKLSKEMVEGMGGYNSPFYQQFKSYCYTTFTALRKS    726
-                      .|..|..::.:.||:     |.|:.|       .::..|:|....||:.
-B1AKP8_HUMAN     578 EKIPFRLTRMLTNAME-----VTGLDG-------NYRITCHTVMEVLREH    615
-VPS34_SCHPO      727 SNLILNLFSLMV----------DAN-------------------------    741
-                     .:.::.:....|          |.|
-B1AKP8_HUMAN     616 KDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDG    665
-VPS34_SCHPO      742 --------------IPD----------IK---FDKE--KVVYKVKERFC-    761
-                                   :|:          :|   .:|:  :::.:|:::..
-B1AKP8_HUMAN     666 VELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTG    715
-VPS34_SCHPO      762 LQMSESDAI---KYFEQLINDSVSALFPQIIDRMHNLAQYMRS-----    801
-                     ...|..|.:   ...|.||..:.|         ..||.|....
-B1AKP8_HUMAN     716 RDFSHDDTLDVPTQVELLIKQATS---------HENLCQCYIGWCPFW    754
 </pre>
@@ Riadok 234: / Riadok 221: @@
 ########################################
 # Program: needle
-# Rundate: Thu 11 Oct 2012 09:37:33
+# Rundate: Thu 14 Oct 2021 15:05:37
 # Commandline: needle
-#    -asequence ggg1
+#    -auto
-#    -bsequence ggg2
+#    -stdout
-#    -outfile ggg12.n
+#    -asequence emboss_needle-I20211014-151156-0154-17922699-p1m.asequence
-# Align_format: srspair
+#    -bsequence emboss_needle-I20211014-151156-0154-17922699-p1m.bsequence
-# Report_file: ggg12.n
+#    -datafile EBLOSUM62
+#    -gapopen 10.0
+#    -gapextend 0.5
+#    -endopen 10.0
+#    -endextend 0.5
+#    -aformat3 pair
+#    -sprotein1
+#    -sprotein2
+# Align_format: pair
+# Report_file: stdout
 ########################################
@@ Riadok 246: / Riadok 242: @@
 #
 # Aligned_sequences: 2
-# 1: VPS34_SCHPO
+# 1: MCA_00027_1
-# 2: B1AKP8_HUMAN
+# 2: RKM3_YEAST
 # Matrix: EBLOSUM62
 # Gap_penalty: 10.0
 # Extend_penalty: 0.5
 #
-# Length: 1060
+# Length: 651
-# Identity:     138/1060 (13.0%)
+# Identity:     177/651 (27.2%)
-# Similarity:   245/1060 (23.1%)
+# Similarity:   282/651 (43.3%)
-# Gaps:         565/1060 (53.3%)
+# Gaps:         155/651 (23.8%)
-# Score: 206.0
+# Score: 608.0
 #
 #
 #=======================================
-VPS34_SCHPO        1 MDRLVFSYCPSSKVTARFLVKFCFIEYQDSQEPCICTIQLFSGNESGSLM     50
+MCA_00027_1        1 ----MEDSLMERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFAR     42
+                         .:|. :.|...||    |...|:|..      ||||||||:||||:
-B1AKP8_HUMAN       0 --------------------------------------------------      0
+RKM3_YEAST         1 MSVTFKDD-VHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAK     43
-VPS34_SCHPO       51 QKCFVSKIPNKSLLPTELSKISTHEWLDFGVTVSELSLNAKFVVSAWKPS    100
-B1AKP8_HUMAN       0 --------------------------------------------------      0
-VPS34_SCHPO      101 FNDEEVYEFVGCTTYRLFDENNLLRQGLQKIPLQTSKEIKKYSPTSLELE    150
-B1AKP8_HUMAN       0 --------------------------------------------------      0
-VPS34_SCHPO      151 QVKEINRLDGLLLKLQLGDVPSVNWLDDISFGKIKDFRSKHMSLVTIPIL    200
-B1AKP8_HUMAN       0 --------------------------------------------------      0
-VPS34_SCHPO      201 YLDFLQFSFPVVFQRSYYPKSENRVYYSSFDLELNLDSPAELKHRRLVRS    250
-                                                      :|.::....||:...|.
-B1AKP8_HUMAN       1 ---------------------------------MNFEAVLHYKHQNQARD     17
-VPS34_SCHPO      251 QRNGPLDKDLK--------------------------PNSKIRKELESIL    274
-                     ::     |.|:                          ..|....|.||..
-B1AKP8_HUMAN      18 EK-----KKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTE     62
-VPS34_SCHPO      275 SYP-PSEELSLEEKDLIWKFRFYLTRNKKAMTKFLKSVVWTDSSEVNQAL    323
-                     :.| ||.......:||......|..   .|:..|.:|:..:..:.:...|
-B1AKP8_HUMAN      63 NSPTPSPLQKKVTEDLSKTLLMYTV---PAVQGFFRSISLSRGNNLQDTL    109
-VPS34_SCHPO      324 SLLDSWTEI----DIDDALELLSPSFVHPKVRAYAVSRLETASNEELLLY    369
-                     .:|..|.:.    |:::||           |......:::|
-B1AKP8_HUMAN     110 RVLTLWFDYGHWPDVNEAL-----------VEGVKAIQIDT---------    139
-VPS34_SCHPO      370 LLQLVQAL--RYDNP-----------ISSDERFQPS----PLAL------    396
-                     .||::..|  |.|.|           ::...|:.|.    ||.:
-B1AKP8_HUMAN     140 WLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTT    189
-VPS34_SCHPO      397 -------------------FLVNRA--ISSPSIGNDLYWYLVVEIEDEPV    425
+MCA_00027_1       43 KKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSL     92
-                                        .||.:|  :|...|...:.|:.:.....|..
+                     ..|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.::
-B1AKP8_HUMAN     190 TARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEA    239
+RKM3_YEAST        44 TDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTV     93
-VPS34_SCHPO      426 SKLF---SSVMFLFQ----------------KELS---------------    441
+MCA_00027_1       93 GPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL--    136
-                      |:|:   .:|..:|:                ||.|
+                      ..:|. ||.|::||   |.:..::..|.||..:.:.||.||.  :.||
-B1AKP8_HUMAN     240 SRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEW    289
+RKM3_YEAST        94 FRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFD    141
-VPS34_SCHPO      442 --KSVEGRLIRETLSAQAKFVEKLLRISKSVQSFRGTRLKKIEYLKVLLE    489
+MCA_00027_1      137 -KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQY    167
-                       |.::...:::...|...:.....||||.:.......|:.:....::..
+                      ...:::|.|.|:..|                  |...|.||    :..|
-B1AKP8_HUMAN     290 CRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCR    339
+RKM3_YEAST       142 SLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDY    187
-VPS34_SCHPO      490 DHKYHLLDFHALPLPLDPSVNIVGI--IPDACTVFKSTMQPLRLLFKCQD    537
+MCA_00027_1      168 R-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVV    215
-                      |.:.      |:|...||:..|:.|  |..:..|..|..:|.:|.....:
+                      . .||.:......::||.|::|.||..||||.|.||||.: |..:.|..:
-B1AKP8_HUMAN     340 DLEL------AVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSN    383
+RKM3_YEAST       188 NLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSL    237
-VPS34_SCHPO      538 GSKYPIIFKNGDDLRQDQLVIQILTLMDKLLKKE----KLDLHLKPYRIL    583
+MCA_00027_1      216 SQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYS    265
-                      |.::..:.|..:|||||:.|:|:..|::.||..:    :.:|.::.|.::
+                      ..||..||.||.|.||.        ::|..|.|.:|:....
-B1AKP8_HUMAN     384 GHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVI    433
+RKM3_YEAST       238 YDVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK---------    270
-VPS34_SCHPO      584 ATGPTHGAVQFVP-SKTLATILAEYHG-----------------------    609
+MCA_00027_1      266 DSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEA    315
-                     ......|.:.:|| ..||..::.:|..
+                                          :.|.||.: |.||..|...|:.:.|....
-B1AKP8_HUMAN     434 PLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHL    483
+RKM3_YEAST       271 ---------------------VASMETRV-IDEDLIKSLENDLEKEYSNV    298
-VPS34_SCHPO      610 ------SVLAYLRENNPDDGLNSANYGIDP---VAMD---NYVRSCAGYC    647
+MCA_00027_1      316 EKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLAS    365
-                           .|..:...|...|.|....:...|   |..|   ||.||.|...
+                     .    ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.:
-B1AKP8_HUMAN     484 TLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMS    533
+RKM3_YEAST       299 T----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLA    344
-VPS34_SCHPO      648 VITYLLGVGDRHLDNLLITK-DGHFFHADFG--------------YILGR    682
+MCA_00027_1      366 RYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRK    414
-                      ::.|:||:||||..||::.: .|...|.|||              .|..|
+                      ||||.:.:|:|:.|.|.|:..:.: .|....|:.:|||    :...|:..
-B1AKP8_HUMAN     534 MVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFR    583
+RKM3_YEAST       345 RYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFS    390
-VPS34_SCHPO      683 DPKLFSPAMKLSKEMVEGMGGYNSPFYQQFKSYCYTTFTALRKSSNLILN    732
+MCA_00027_1      415 DEWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDE    452
-                      ..::.:.||:     |.|:.|       .::..|:|....||:..:.::.
+                      ..:|::.:.::||::|            ||:.|||.....|.|  ||..:
-B1AKP8_HUMAN     584 LTRMLTNAME-----VTGLDG-------NYRITCHTVMEVLREHKDSVMA    621
+RKM3_YEAST       391 AWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQ    438
-VPS34_SCHPO      733 LFSLMV----------DANIPDIKFDKEKVVYKVKERFCLQMSESDAIKY    772
+MCA_00027_1      453 AYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STL    501
-                      :....|          |.|....|..:.:
+                      .|:.:||.||.....|..:|:::...:|:|        |:.|..|. |..
-B1AKP8_HUMAN     622 VLEAFVYDPLLNWRLMDTNTKGNKRSRTR---------------------    650
+RKM3_YEAST       439 LYIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDS    480
-VPS34_SCHPO      773 FEQLINDSVSA-LFPQIIDRMHNLAQYMRS--------------------    801
+MCA_00027_1      502 VFNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQK    544
-                          .||.|| ...:|:|.: .|.:....
+                     :.||:.|..|....|...::....:|...|:       ...||..:.:..
-B1AKP8_HUMAN     651 -----TDSYSAGQSVEILDGV-ELGEPAHKKTGTTVPESIHSFIGDGLVK    694
+RKM3_YEAST       481 IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSML    530
-VPS34_SCHPO      801 --------------------------------------------------    801
+MCA_00027_1      545 KNSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKL    594
+                     :|:|     |:..:|..:|::.::.::
-B1AKP8_HUMAN     695 PEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLC    744
+RKM3_YEAST       531 QNAR-----TLVQSEHNILDRCLKRLS-----------------------    552
-VPS34_SCHPO      801 ----------    801
+MCA_00027_1      595 K    595
-B1AKP8_HUMAN     745 QCYIGWCPFW    754
+RKM3_YEAST       553 -    552
 </pre>