2-AIN-505, 2-AIN-251: Seminár z bioinformatiky (1) a (3)
Zima 2019

Jared T. Simpson, Rachael E. Workman, P. C. Zuzarte, Matei David, L. J. Dursi, Winston Timp. Detecting DNA cytosine methylation using nanopore sequencing. Nature methods, 14(4):407-410. 2017. Supplement https://media.nature.com/original/nature-assets/nmeth/journal/v14/n4/extref/nmeth.4184-S1.pdf.

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Bibliography entry: BibTeX


In nanopore sequencing devices, electrolytic current signals are sensitive to
base modifications, such as 5-methylcytosine (5-mC). Here we quantified the
strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By
using synthetically methylated DNA, we were able to train a hidden Markov model
to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence
the methylome of human DNA, without requiring special steps for library