2-AIN-506 a 2-AIN-252: Seminár z bioinformatiky (2) a (4)
Leto 2018

Arthur C. Rand, Miten Jain, Jordan M. Eizenga, Audrey Musselman-Brown, Hugh E. Olsen, Mark Akeson, Benedict Paten. Mapping DNA methylation with high-throughput nanopore sequencing. Nature methods, 14(4):411-413. 2017.

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Related web page: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704956/

Bibliography entry: BibTeX


DNA chemical modifications regulate genomic function. We present a framework for 
mapping cytosine and adenosine methylation with the Oxford Nanopore Technologies 
MinION using this nanopore sequencer's ionic current signal. We map three
cytosine variants and two adenine variants. The results show that our model is
sensitive enough to detect changes in genomic DNA methylation levels as a
function of growth phase in Escherichia coli.