CB-aln-dp: Rozdiel medzi revíziami
Z MBI
Riadok 21: | Riadok 21: | ||
<pre> | <pre> | ||
− | > | + | >RKM3_YEAST |
− | + | MSVTFKDDVHRILKFVANCNGRFEDSKCDIRESPLGGLGVFAKTDIAEGESILTLNKSSI | |
− | + | FSASNSSIANLLCDSSIDGMLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPP | |
− | + | SFWHADAKRLLKGTSFDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSE | |
− | + | ENHEEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLYDV | |
− | + | CDKCGEPDMCKHLIAEEYLEAENLDKNMPKVASMETRVIDEDLIKSLENDLEKEYSNVTA | |
− | + | NIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLARYGFTVPENQYDIVHL | |
− | + | GPDFMKILKKEEKYQEKVKWWSQVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESE | |
− | + | EEEEEEEGDDSLESWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESLFSKKATPHISDS | |
− | + | IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQNARTLVQSE | |
− | + | HNILDRCLKRLS | |
− | + | ||
− | + | ||
− | + | ||
</pre> | </pre> | ||
Riadok 41: | Riadok 38: | ||
######################################## | ######################################## | ||
# Program: water | # Program: water | ||
− | # Rundate: Thu | + | # Rundate: Thu 14 Oct 2021 15:08:56 |
# Commandline: water | # Commandline: water | ||
− | # -asequence | + | # -auto |
− | # -bsequence | + | # -stdout |
− | # - | + | # -asequence emboss_water-I20211014-150851-0938-20653107-p2m.asequence |
− | # Align_format: | + | # -bsequence emboss_water-I20211014-150851-0938-20653107-p2m.bsequence |
− | # Report_file: | + | # -datafile EBLOSUM62 |
+ | # -gapopen 10.0 | ||
+ | # -gapextend 0.5 | ||
+ | # -aformat3 pair | ||
+ | # -sprotein1 | ||
+ | # -sprotein2 | ||
+ | # Align_format: pair | ||
+ | # Report_file: stdout | ||
######################################## | ######################################## | ||
Riadok 53: | Riadok 57: | ||
# | # | ||
# Aligned_sequences: 2 | # Aligned_sequences: 2 | ||
− | # 1: | + | # 1: MCA_00027_1 |
− | # 2: | + | # 2: RKM3_YEAST |
# Matrix: EBLOSUM62 | # Matrix: EBLOSUM62 | ||
# Gap_penalty: 10.0 | # Gap_penalty: 10.0 | ||
# Extend_penalty: 0.5 | # Extend_penalty: 0.5 | ||
# | # | ||
− | # Length: | + | # Length: 588 |
− | # Identity: | + | # Identity: 170/588 (28.9%) |
− | # Similarity: | + | # Similarity: 270/588 (45.9%) |
− | # Gaps: | + | # Gaps: 116/588 (19.7%) |
− | # Score: | + | # Score: 611.0 |
# | # | ||
# | # | ||
#======================================= | #======================================= | ||
− | + | MCA_00027_1 29 IRESPLGGIGVFARKKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDG 78 | |
− | | | + | ||||||||:||||:..|.:...||.:.|:.|.|.||.||:|||.::..|| |
− | + | RKM3_YEAST 30 IRESPLGGLGVFAKTDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDG 79 | |
− | + | MCA_00027_1 79 MLGLALAYMYERSLGPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEE 124 | |
− | + | ||.|.:|::||.::..:|. ||.|::|| |.:..::..|.||..:.:. | |
− | + | RKM3_YEAST 80 MLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKR 129 | |
− | + | MCA_00027_1 125 LLVGTELYYHTL---KVEDDDIAEVFKFDV------------------LP 153 | |
− | + | ||.||. :.|| ...:::|.|.|:..| |. | |
− | + | RKM3_YEAST 130 LLKGTS--FDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLD 177 | |
− | + | MCA_00027_1 154 FLERNHLFEGQPQYR-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLF 202 | |
− | .|.| | + | ..|.|| :..|. .||.:......::||.|::|.||..||||.|.|| |
− | + | RKM3_YEAST 178 VSEENH----EEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLF 223 | |
− | + | MCA_00027_1 203 NHSI-DESVHFEVVSQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVD 251 | |
− | + | ||.: |..:.|..:..||..||.||.|.||. ::|..|.|.:| | |
− | + | RKM3_YEAST 224 NHHVSDPDLKFVSLYDVCDKCGEPDMCKHLI--------AEEYLEAENLD 265 | |
− | + | MCA_00027_1 252 ELNEDGFSSGFDYSDSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSD 301 | |
− | + | :.... :.|.||.: |.||.. | |
− | + | RKM3_YEAST 266 KNMPK------------------------------VASMETRV-IDEDLI 284 | |
− | + | MCA_00027_1 302 KKTSNEQKSEEQEAEKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEV 351 | |
− | + | |...|:.:.|..... ...::|:...:.|.:..|:|....|.:|.|: | |
− | + | RKM3_YEAST 285 KSLENDLEKEYSNVT----ANIEDDDGGIENPDECVDLVLKNDVAQGQEI 330 | |
− | + | MCA_00027_1 352 FNTYGELSNHHLASRYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEW 400 | |
− | :| | + | ||:||||||..|.:||||.:.:|:|:.|.|.|:..:.: .|....|:.:| |
− | + | RKM3_YEAST 331 FNSYGELSNVFLLARYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKW 380 | |
− | + | MCA_00027_1 401 WSIYFYEALFGIRKDEWAEIEESEDEDDEG------------SEDSEEEN 438 | |
− | + | || :...|:....:|::.:.::||::| ||:.|||. | |
− | + | RKM3_YEAST 381 WS----QVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEE 426 | |
− | + | MCA_00027_1 439 SIPPSPESISWEDEAYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFER 488 | |
− | + | ....|.| ||..:.|:.:||.||.....|..:|:::...:|:| | |
− | + | RKM3_YEAST 427 EGDDSLE--SWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESL------ 468 | |
− | + | MCA_00027_1 489 DVFTSKLLPS-STLVFNEKSLILLKALVNLRLQRYKDGMLTSSQI----- 532 | |
− | + | |:.|..|. |..:.||:.|..|....|...::....:|...|: | |
− | + | RKM3_YEAST 469 --FSKKATPHISDSIVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKG 516 | |
− | + | MCA_00027_1 533 --KDLITKLSDNQKKNSRQILALTIKGTEKIVLEKSMQ 568 | |
− | + | ...||..:.:..:|:| |:..:|..:|::.:: | |
− | + | RKM3_YEAST 517 DNSSKITSATKSMLQNAR-----TLVQSEHNILDRCLK 549 | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</pre> | </pre> | ||
Riadok 125: | Riadok 125: | ||
######################################## | ######################################## | ||
# Program: needle | # Program: needle | ||
− | # Rundate: Thu | + | # Rundate: Thu 14 Oct 2021 15:13:34 |
# Commandline: needle | # Commandline: needle | ||
− | # -asequence | + | # -auto |
− | # -bsequence | + | # -stdout |
+ | # -asequence emboss_needle-I20211014-151332-0965-44565643-p2m.asequence | ||
+ | # -bsequence emboss_needle-I20211014-151332-0965-44565643-p2m.bsequence | ||
+ | # -datafile EBLOSUM62 | ||
+ | # -gapopen 10.0 | ||
+ | # -gapextend 0.5 | ||
# -endweight | # -endweight | ||
− | # - | + | # -endopen 10.0 |
− | # Align_format: | + | # -endextend 0.5 |
− | # Report_file: | + | # -aformat3 pair |
+ | # -sprotein1 | ||
+ | # -sprotein2 | ||
+ | # Align_format: pair | ||
+ | # Report_file: stdout | ||
######################################## | ######################################## | ||
Riadok 138: | Riadok 147: | ||
# | # | ||
# Aligned_sequences: 2 | # Aligned_sequences: 2 | ||
− | # 1: | + | # 1: MCA_00027_1 |
− | # 2: | + | # 2: RKM3_YEAST |
# Matrix: EBLOSUM62 | # Matrix: EBLOSUM62 | ||
# Gap_penalty: 10.0 | # Gap_penalty: 10.0 | ||
# Extend_penalty: 0.5 | # Extend_penalty: 0.5 | ||
# | # | ||
− | # Length: | + | # Length: 650 |
− | # Identity: | + | # Identity: 178/650 (27.4%) |
− | # Similarity: | + | # Similarity: 282/650 (43.4%) |
− | # Gaps: | + | # Gaps: 153/650 (23.5%) |
− | # Score: | + | # Score: 588.5 |
# | # | ||
# | # | ||
#======================================= | #======================================= | ||
− | + | MCA_00027_1 1 MEDSL---MERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFARK 43 | |
− | |: ..|..| | + | |..:. :.|...|| |...|:|.. ||||||||:||||:. |
− | + | RKM3_YEAST 1 MSVTFKDDVHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAKT 44 | |
− | + | MCA_00027_1 44 KIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSLG 93 | |
− | + | .|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.::. | |
− | + | RKM3_YEAST 45 DIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTVF 94 | |
− | + | MCA_00027_1 94 PDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL--- 136 | |
− | . | + | .:|. ||.|::|| |.:..::..|.||..:.:.||.||. :.|| |
− | + | RKM3_YEAST 95 RNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFDS 142 | |
− | + | MCA_00027_1 137 KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQYR 168 | |
− | + | ...:::|.|.|:..| |...|.|| :..|. | |
− | + | RKM3_YEAST 143 LAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDYN 188 | |
− | + | MCA_00027_1 169 -TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVVS 216 | |
− | + | .||.:......::||.|::|.||..||||.|.||||.: |..:.|..:. | |
− | + | RKM3_YEAST 189 LKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLY 238 | |
− | + | MCA_00027_1 217 QVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYSD 266 | |
− | ..|.| | + | .||..||.||.|.||. ::|..|.|.:|:.... |
− | + | RKM3_YEAST 239 DVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK---------- 270 | |
− | + | MCA_00027_1 267 SESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEAE 316 | |
− | + | :.|.||.: |.||..|...|:.:.|..... | |
− | + | RKM3_YEAST 271 --------------------VASMETRV-IDEDLIKSLENDLEKEYSNVT 299 | |
− | + | MCA_00027_1 317 KYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLASR 366 | |
− | + | ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.:| | |
− | + | RKM3_YEAST 300 ----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLAR 345 | |
− | + | MCA_00027_1 367 YGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRKD 415 | |
− | + | |||.:.:|:|:.|.|.|:..:.: .|....|:.:||| :...|:... | |
− | + | RKM3_YEAST 346 YGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFSA 391 | |
− | + | MCA_00027_1 416 EWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDEA 453 | |
− | + | .:|::.:.::||::| ||:.|||.....|.| ||..:. | |
− | + | RKM3_YEAST 392 WYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQL 439 | |
− | + | MCA_00027_1 454 YLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STLV 502 | |
− | + | |:.:||.||.....|..:|:::...:|:| |:.|..|. |..: | |
− | + | RKM3_YEAST 440 YIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDSI 481 | |
− | + | MCA_00027_1 503 FNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQKK 545 | |
− | .... | + | .||:.|..|....|...::....:|...|: ...||..:.:..: |
− | + | RKM3_YEAST 482 VNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQ 531 | |
− | + | MCA_00027_1 546 NSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKLK 595 | |
− | + | |:| |:..:|..:|::.: ::|. | |
− | + | RKM3_YEAST 532 NAR-----TLVQSEHNILDRCL------------------------KRLS 552 | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
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− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</pre> | </pre> | ||
Riadok 234: | Riadok 219: | ||
######################################## | ######################################## | ||
# Program: needle | # Program: needle | ||
− | # Rundate: Thu | + | # Rundate: Thu 14 Oct 2021 15:05:37 |
# Commandline: needle | # Commandline: needle | ||
− | # -asequence | + | # -auto |
− | # -bsequence | + | # -stdout |
− | # - | + | # -asequence emboss_needle-I20211014-151156-0154-17922699-p1m.asequence |
− | # Align_format: | + | # -bsequence emboss_needle-I20211014-151156-0154-17922699-p1m.bsequence |
− | # Report_file: | + | # -datafile EBLOSUM62 |
+ | # -gapopen 10.0 | ||
+ | # -gapextend 0.5 | ||
+ | # -endopen 10.0 | ||
+ | # -endextend 0.5 | ||
+ | # -aformat3 pair | ||
+ | # -sprotein1 | ||
+ | # -sprotein2 | ||
+ | # Align_format: pair | ||
+ | # Report_file: stdout | ||
######################################## | ######################################## | ||
Riadok 246: | Riadok 240: | ||
# | # | ||
# Aligned_sequences: 2 | # Aligned_sequences: 2 | ||
− | # 1: | + | # 1: MCA_00027_1 |
− | # 2: | + | # 2: RKM3_YEAST |
# Matrix: EBLOSUM62 | # Matrix: EBLOSUM62 | ||
# Gap_penalty: 10.0 | # Gap_penalty: 10.0 | ||
# Extend_penalty: 0.5 | # Extend_penalty: 0.5 | ||
# | # | ||
− | # Length: | + | # Length: 651 |
− | # Identity: | + | # Identity: 177/651 (27.2%) |
− | # Similarity: | + | # Similarity: 282/651 (43.3%) |
− | # Gaps: | + | # Gaps: 155/651 (23.8%) |
− | # Score: | + | # Score: 608.0 |
# | # | ||
# | # | ||
#======================================= | #======================================= | ||
− | + | MCA_00027_1 1 ----MEDSLMERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFAR 42 | |
− | + | .:|. :.|...|| |...|:|.. ||||||||:||||: | |
− | + | RKM3_YEAST 1 MSVTFKDD-VHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAK 43 | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | MCA_00027_1 43 KKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSL 92 | |
− | + | ..|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.:: | |
− | + | RKM3_YEAST 44 TDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTV 93 | |
− | + | MCA_00027_1 93 GPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL-- 136 | |
− | + | ..:|. ||.|::|| |.:..::..|.||..:.:.||.||. :.|| | |
− | + | RKM3_YEAST 94 FRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFD 141 | |
− | + | MCA_00027_1 137 -KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQY 167 | |
− | + | ...:::|.|.|:..| |...|.|| :..| | |
− | + | RKM3_YEAST 142 SLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDY 187 | |
− | + | MCA_00027_1 168 R-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVV 215 | |
− | + | . .||.:......::||.|::|.||..||||.|.||||.: |..:.|..: | |
− | + | RKM3_YEAST 188 NLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSL 237 | |
− | + | MCA_00027_1 216 SQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYS 265 | |
− | + | ..||..||.||.|.||. ::|..|.|.:|:.... | |
− | + | RKM3_YEAST 238 YDVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK--------- 270 | |
− | + | MCA_00027_1 266 DSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEA 315 | |
− | + | :.|.||.: |.||..|...|:.:.|.... | |
− | + | RKM3_YEAST 271 ---------------------VASMETRV-IDEDLIKSLENDLEKEYSNV 298 | |
− | + | MCA_00027_1 316 EKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLAS 365 | |
− | + | . ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.: | |
− | + | RKM3_YEAST 299 T----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLA 344 | |
− | + | MCA_00027_1 366 RYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRK 414 | |
− | + | ||||.:.:|:|:.|.|.|:..:.: .|....|:.:||| :...|:.. | |
− | + | RKM3_YEAST 345 RYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFS 390 | |
− | + | MCA_00027_1 415 DEWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDE 452 | |
− | ..::.:.||: | + | ..:|::.:.::||::| ||:.|||.....|.| ||..: |
− | + | RKM3_YEAST 391 AWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQ 438 | |
− | + | MCA_00027_1 453 AYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STL 501 | |
− | :. | + | .|:.:||.||.....|..:|:::...:|:| |:.|..|. |.. |
− | + | RKM3_YEAST 439 LYIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDS 480 | |
− | + | MCA_00027_1 502 VFNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQK 544 | |
− | + | :.||:.|..|....|...::....:|...|: ...||..:.:.. | |
− | + | RKM3_YEAST 481 IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSML 530 | |
− | + | MCA_00027_1 545 KNSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKL 594 | |
− | + | :|:| |:..:|..:|::.::.:: | |
− | + | RKM3_YEAST 531 QNAR-----TLVQSEHNILDRCLKRLS----------------------- 552 | |
− | + | MCA_00027_1 595 K 595 | |
− | + | ||
− | + | RKM3_YEAST 553 - 552 | |
</pre> | </pre> |
Verzia zo dňa a času 15:23, 14. október 2021
Sekvencie a zarovnania k cvičeniu pre biológov: programy water a needle
Obsah
Proteínové sekvencie
>sp|P50520|VPS34_SCHPO Phosphatidylinositol 3-kinase vps34 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=vps34 PE=2 SV=2 MDRLVFSYCPSSKVTARFLVKFCFIEYQDSQEPCICTIQLFSGNESGSLMQKCFVSKIPN KSLLPTELSKISTHEWLDFGVTVSELSLNAKFVVSAWKPSFNDEEVYEFVGCTTYRLFDE NNLLRQGLQKIPLQTSKEIKKYSPTSLELEQVKEINRLDGLLLKLQLGDVPSVNWLDDIS FGKIKDFRSKHMSLVTIPILYLDFLQFSFPVVFQRSYYPKSENRVYYSSFDLELNLDSPA ELKHRRLVRSQRNGPLDKDLKPNSKIRKELESILSYPPSEELSLEEKDLIWKFRFYLTRN KKAMTKFLKSVVWTDSSEVNQALSLLDSWTEIDIDDALELLSPSFVHPKVRAYAVSRLET ASNEELLLYLLQLVQALRYDNPISSDERFQPSPLALFLVNRAISSPSIGNDLYWYLVVEI EDEPVSKLFSSVMFLFQKELSKSVEGRLIRETLSAQAKFVEKLLRISKSVQSFRGTRLKK IEYLKVLLEDHKYHLLDFHALPLPLDPSVNIVGIIPDACTVFKSTMQPLRLLFKCQDGSK YPIIFKNGDDLRQDQLVIQILTLMDKLLKKEKLDLHLKPYRILATGPTHGAVQFVPSKTL ATILAEYHGSVLAYLRENNPDDGLNSANYGIDPVAMDNYVRSCAGYCVITYLLGVGDRHL DNLLITKDGHFFHADFGYILGRDPKLFSPAMKLSKEMVEGMGGYNSPFYQQFKSYCYTTF TALRKSSNLILNLFSLMVDANIPDIKFDKEKVVYKVKERFCLQMSESDAIKYFEQLINDS VSALFPQIIDRMHNLAQYMRS
>RKM3_YEAST MSVTFKDDVHRILKFVANCNGRFEDSKCDIRESPLGGLGVFAKTDIAEGESILTLNKSSI FSASNSSIANLLCDSSIDGMLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPP SFWHADAKRLLKGTSFDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSE ENHEEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLYDV CDKCGEPDMCKHLIAEEYLEAENLDKNMPKVASMETRVIDEDLIKSLENDLEKEYSNVTA NIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLARYGFTVPENQYDIVHL GPDFMKILKKEEKYQEKVKWWSQVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESE EEEEEEEGDDSLESWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESLFSKKATPHISDS IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQNARTLVQSE HNILDRCLKRLS
Lokálne zarovnanie
######################################## # Program: water # Rundate: Thu 14 Oct 2021 15:08:56 # Commandline: water # -auto # -stdout # -asequence emboss_water-I20211014-150851-0938-20653107-p2m.asequence # -bsequence emboss_water-I20211014-150851-0938-20653107-p2m.bsequence # -datafile EBLOSUM62 # -gapopen 10.0 # -gapextend 0.5 # -aformat3 pair # -sprotein1 # -sprotein2 # Align_format: pair # Report_file: stdout ######################################## #======================================= # # Aligned_sequences: 2 # 1: MCA_00027_1 # 2: RKM3_YEAST # Matrix: EBLOSUM62 # Gap_penalty: 10.0 # Extend_penalty: 0.5 # # Length: 588 # Identity: 170/588 (28.9%) # Similarity: 270/588 (45.9%) # Gaps: 116/588 (19.7%) # Score: 611.0 # # #======================================= MCA_00027_1 29 IRESPLGGIGVFARKKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDG 78 ||||||||:||||:..|.:...||.:.|:.|.|.||.||:|||.::..|| RKM3_YEAST 30 IRESPLGGLGVFAKTDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDG 79 MCA_00027_1 79 MLGLALAYMYERSLGPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEE 124 ||.|.:|::||.::..:|. ||.|::|| |.:..::..|.||..:.:. RKM3_YEAST 80 MLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKR 129 MCA_00027_1 125 LLVGTELYYHTL---KVEDDDIAEVFKFDV------------------LP 153 ||.||. :.|| ...:::|.|.|:..| |. RKM3_YEAST 130 LLKGTS--FDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLD 177 MCA_00027_1 154 FLERNHLFEGQPQYR-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLF 202 ..|.|| :..|. .||.:......::||.|::|.||..||||.|.|| RKM3_YEAST 178 VSEENH----EEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLF 223 MCA_00027_1 203 NHSI-DESVHFEVVSQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVD 251 ||.: |..:.|..:..||..||.||.|.||. ::|..|.|.:| RKM3_YEAST 224 NHHVSDPDLKFVSLYDVCDKCGEPDMCKHLI--------AEEYLEAENLD 265 MCA_00027_1 252 ELNEDGFSSGFDYSDSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSD 301 :.... :.|.||.: |.||.. RKM3_YEAST 266 KNMPK------------------------------VASMETRV-IDEDLI 284 MCA_00027_1 302 KKTSNEQKSEEQEAEKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEV 351 |...|:.:.|..... ...::|:...:.|.:..|:|....|.:|.|: RKM3_YEAST 285 KSLENDLEKEYSNVT----ANIEDDDGGIENPDECVDLVLKNDVAQGQEI 330 MCA_00027_1 352 FNTYGELSNHHLASRYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEW 400 ||:||||||..|.:||||.:.:|:|:.|.|.|:..:.: .|....|:.:| RKM3_YEAST 331 FNSYGELSNVFLLARYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKW 380 MCA_00027_1 401 WSIYFYEALFGIRKDEWAEIEESEDEDDEG------------SEDSEEEN 438 || :...|:....:|::.:.::||::| ||:.|||. RKM3_YEAST 381 WS----QVGHGLFSAWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEE 426 MCA_00027_1 439 SIPPSPESISWEDEAYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFER 488 ....|.| ||..:.|:.:||.||.....|..:|:::...:|:| RKM3_YEAST 427 EGDDSLE--SWLSQLYIDSSGEPSPSTWALANLLTLTAVQWESL------ 468 MCA_00027_1 489 DVFTSKLLPS-STLVFNEKSLILLKALVNLRLQRYKDGMLTSSQI----- 532 |:.|..|. |..:.||:.|..|....|...::....:|...|: RKM3_YEAST 469 --FSKKATPHISDSIVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKG 516 MCA_00027_1 533 --KDLITKLSDNQKKNSRQILALTIKGTEKIVLEKSMQ 568 ...||..:.:..:|:| |:..:|..:|::.:: RKM3_YEAST 517 DNSSKITSATKSMLQNAR-----TLVQSEHNILDRCLK 549
Globálne zarovnanie
######################################## # Program: needle # Rundate: Thu 14 Oct 2021 15:13:34 # Commandline: needle # -auto # -stdout # -asequence emboss_needle-I20211014-151332-0965-44565643-p2m.asequence # -bsequence emboss_needle-I20211014-151332-0965-44565643-p2m.bsequence # -datafile EBLOSUM62 # -gapopen 10.0 # -gapextend 0.5 # -endweight # -endopen 10.0 # -endextend 0.5 # -aformat3 pair # -sprotein1 # -sprotein2 # Align_format: pair # Report_file: stdout ######################################## #======================================= # # Aligned_sequences: 2 # 1: MCA_00027_1 # 2: RKM3_YEAST # Matrix: EBLOSUM62 # Gap_penalty: 10.0 # Extend_penalty: 0.5 # # Length: 650 # Identity: 178/650 (27.4%) # Similarity: 282/650 (43.4%) # Gaps: 153/650 (23.5%) # Score: 588.5 # # #======================================= MCA_00027_1 1 MEDSL---MERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFARK 43 |..:. :.|...|| |...|:|.. ||||||||:||||:. RKM3_YEAST 1 MSVTFKDDVHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAKT 44 MCA_00027_1 44 KIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSLG 93 .|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.::. RKM3_YEAST 45 DIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTVF 94 MCA_00027_1 94 PDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL--- 136 .:|. ||.|::|| |.:..::..|.||..:.:.||.||. :.|| RKM3_YEAST 95 RNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFDS 142 MCA_00027_1 137 KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQYR 168 ...:::|.|.|:..| |...|.|| :..|. RKM3_YEAST 143 LAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDYN 188 MCA_00027_1 169 -TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVVS 216 .||.:......::||.|::|.||..||||.|.||||.: |..:.|..:. RKM3_YEAST 189 LKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLY 238 MCA_00027_1 217 QVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYSD 266 .||..||.||.|.||. ::|..|.|.:|:.... RKM3_YEAST 239 DVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK---------- 270 MCA_00027_1 267 SESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEAE 316 :.|.||.: |.||..|...|:.:.|..... RKM3_YEAST 271 --------------------VASMETRV-IDEDLIKSLENDLEKEYSNVT 299 MCA_00027_1 317 KYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLASR 366 ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.:| RKM3_YEAST 300 ----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLAR 345 MCA_00027_1 367 YGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRKD 415 |||.:.:|:|:.|.|.|:..:.: .|....|:.:||| :...|:... RKM3_YEAST 346 YGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFSA 391 MCA_00027_1 416 EWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDEA 453 .:|::.:.::||::| ||:.|||.....|.| ||..:. RKM3_YEAST 392 WYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQL 439 MCA_00027_1 454 YLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STLV 502 |:.:||.||.....|..:|:::...:|:| |:.|..|. |..: RKM3_YEAST 440 YIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDSI 481 MCA_00027_1 503 FNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQKK 545 .||:.|..|....|...::....:|...|: ...||..:.:..: RKM3_YEAST 482 VNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSMLQ 531 MCA_00027_1 546 NSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKLK 595 |:| |:..:|..:|::.: ::|. RKM3_YEAST 532 NAR-----TLVQSEHNILDRCL------------------------KRLS 552
Globálne zarovnanie s nulovou penaltou medzier na koncoch sekvencie
######################################## # Program: needle # Rundate: Thu 14 Oct 2021 15:05:37 # Commandline: needle # -auto # -stdout # -asequence emboss_needle-I20211014-151156-0154-17922699-p1m.asequence # -bsequence emboss_needle-I20211014-151156-0154-17922699-p1m.bsequence # -datafile EBLOSUM62 # -gapopen 10.0 # -gapextend 0.5 # -endopen 10.0 # -endextend 0.5 # -aformat3 pair # -sprotein1 # -sprotein2 # Align_format: pair # Report_file: stdout ######################################## #======================================= # # Aligned_sequences: 2 # 1: MCA_00027_1 # 2: RKM3_YEAST # Matrix: EBLOSUM62 # Gap_penalty: 10.0 # Extend_penalty: 0.5 # # Length: 651 # Identity: 177/651 (27.2%) # Similarity: 282/651 (43.3%) # Gaps: 155/651 (23.8%) # Score: 608.0 # # #======================================= MCA_00027_1 1 ----MEDSLMERYQSFV----GWMLDNKIQFSSLLTIRESPLGGIGVFAR 42 .:|. :.|...|| |...|:|.. ||||||||:||||: RKM3_YEAST 1 MSVTFKDD-VHRILKFVANCNGRFEDSKCD------IRESPLGGLGVFAK 43 MCA_00027_1 43 KKIPKSSLILMVPKNVILSPSNCSISNLLDEADFDGMLGLALAYMYERSL 92 ..|.:...||.:.|:.|.|.||.||:|||.::..||||.|.:|::||.:: RKM3_YEAST 44 TDIAEGESILTLNKSSIFSASNSSIANLLCDSSIDGMLALNIAFIYETTV 93 MCA_00027_1 93 GPDSL-WYEFIQTI---DHDSLISENPRFWPPEDEELLVGTELYYHTL-- 136 ..:|. ||.|::|| |.:..::..|.||..:.:.||.||. :.|| RKM3_YEAST 94 FRNSSHWYPFLRTIRIRDDEGHLNLPPSFWHADAKRLLKGTS--FDTLFD 141 MCA_00027_1 137 -KVEDDDIAEVFKFDV------------------LPFLERNHLFEGQPQY 167 ...:::|.|.|:..| |...|.|| :..| RKM3_YEAST 142 SLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSEENH----EEDY 187 MCA_00027_1 168 R-TLEYYRDCLVAVASRAFDVDVYHGLALVPGACLFNHSI-DESVHFEVV 215 . .||.:......::||.|::|.||..||||.|.||||.: |..:.|..: RKM3_YEAST 188 NLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSL 237 MCA_00027_1 216 SQVCPLCGSPDFCDHLANRLGNHSESDEEEEEEEVDELNEDGFSSGFDYS 265 ..||..||.||.|.||. ::|..|.|.:|:.... RKM3_YEAST 238 YDVCDKCGEPDMCKHLI--------AEEYLEAENLDKNMPK--------- 270 MCA_00027_1 266 DSESDSDGFEDIEEEEEEDGIIGSEETTIPISEDSDKKTSNEQKSEEQEA 315 :.|.||.: |.||..|...|:.:.|.... RKM3_YEAST 271 ---------------------VASMETRV-IDEDLIKSLENDLEKEYSNV 298 MCA_00027_1 316 EKYQYEEDDEDEADYDEPQDTCDIVTIKSVHKGNEVFNTYGELSNHHLAS 365 . ...::|:...:.|.:..|:|....|.:|.|:||:||||||..|.: RKM3_YEAST 299 T----ANIEDDDGGIENPDECVDLVLKNDVAQGQEIFNSYGELSNVFLLA 344 MCA_00027_1 366 RYGFAIWDNKYETVGLSPEIRQYI-SENNLMERQEWWSIYFYEALFGIRK 414 ||||.:.:|:|:.|.|.|:..:.: .|....|:.:||| :...|:.. RKM3_YEAST 345 RYGFTVPENQYDIVHLGPDFMKILKKEEKYQEKVKWWS----QVGHGLFS 390 MCA_00027_1 415 DEWAEIEESEDEDDEG------------SEDSEEENSIPPSPESISWEDE 452 ..:|::.:.::||::| ||:.|||.....|.| ||..: RKM3_YEAST 391 AWYAQMRQEDEEDEDGQAKSDNLSDDIESEEEEEEEEGDDSLE--SWLSQ 438 MCA_00027_1 453 AYLTNSGAPSEGLSKLIRILSMSDSDFEALKSQFERDVFTSKLLPS-STL 501 .|:.:||.||.....|..:|:::...:|:| |:.|..|. |.. RKM3_YEAST 439 LYIDSSGEPSPSTWALANLLTLTAVQWESL--------FSKKATPHISDS 480 MCA_00027_1 502 VFNEKSLILLKALVNLRLQRYKDGMLTSSQI-------KDLITKLSDNQK 544 :.||:.|..|....|...::....:|...|: ...||..:.:.. RKM3_YEAST 481 IVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNSSKITSATKSML 530 MCA_00027_1 545 KNSRQILALTIKGTEKIVLEKSMQWIAQLEKKKKRKPSNSNHKKPNSRKL 594 :|:| |:..:|..:|::.::.:: RKM3_YEAST 531 QNAR-----TLVQSEHNILDRCLKRLS----------------------- 552 MCA_00027_1 595 K 595 RKM3_YEAST 553 - 552