2-AIN-505, 2-AIN-251: Seminár z bioinformatiky (1) a (3)
Zima 2016
Abstrakt

Matthew Loose, Sunir Malla, Michael Stout. Real-time selective sequencing using nanopore technology. Nature methods, 13(9):751-754. 2016.

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Abstract:

The Oxford Nanopore Technologies MinION sequencer enables the selection of
specific DNA molecules for sequencing by reversing the driving voltage across
individual nanopores. To directly select molecules for sequencing, we used
dynamic time warping to match reads to reference sequences. We demonstrate our
open-source Read Until software in real-time selective sequencing of regions
within small genomes, individual amplicon enrichment and normalization of an
amplicon set.