2-AIN-505, 2-AIN-251: Seminár z bioinformatiky (1) a (3)
Zima 2015
Abstrakt

Nicholas J. Loman, Joshua Quick, Jared T. Simpson. A complete bacterial genome assembled de novo using only nanopore sequencingdata. Nature methods, 12(8):733-735. 2015.

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Abstract:

We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single
4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps
are detected between reads and then corrected by a multiple-alignment process;
(ii) corrected reads are assembled using the Celera Assembler; and (iii) the
assembly is polished using a probabilistic model of the signal-level data. The
assembly reconstructs gene order and has 99.5% nucleotide identity.