2-AIN-505, 2-AIN-251: Seminár z bioinformatiky (1) a (3)
Zima 2016
Abstrakt

Nathan J. Kuwada, Beth Traxler, Paul A. Wiggins. Genome-scale quantitative characterization of bacterial protein localization dynamics throughout the cell cycle. Molecular microbiology, 95(1):64-79. 2015.

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Download from publisher: http://onlinelibrary.wiley.com/doi/10.1111/mmi.12841/pdf PubMed

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Abstract:

Bacterial cells display both spatial and temporal organization, and this complex 
structure is known to play a central role in cellular function. Although nearly
one-fifth of all proteins in Escherichia coli localize to specific subcellular
locations, fundamental questions remain about how cellular-scale structure is
encoded at the level of molecular-scale interactions. One significant limitation 
to our understanding is that the localization behavior of only a small subset of 
proteins has been characterized in detail. As an essential step toward a global
model of protein localization in bacteria, we capture and quantitatively analyze 
spatial and temporal protein localization patterns throughout the cell cycle for 
nearly every protein in E. coli that exhibits nondiffuse localization. This
genome-scale analysis reveals significant complexity in patterning, notably in
the behavior of DNA-binding proteins. Complete cell-cycle imaging also
facilitates analysis of protein partitioning to daughter cells at division,
revealing a broad and robust assortment of asymmetric partitioning behaviors.