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Bratislava Biocenter

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The MGC Project Team. The completion of the Mammalian Gene Collection (MGC). Genome research, 19(12):2324-2333. 2009.

Download preprint: not available

Download from publisher: http://dx.doi.org/10.1101/gr.095976.109

Related web page: not available

Bibliography entry: BibTeX

Abstract: 

Since its start, the Mammalian Gene Collection (MGC) has sought to provide
at least one full-protein-coding sequence cDNA clone for every human and
mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC
cloning effort initially relied on random expressed sequence tag screening
of cDNA libraries. Here, we summarize our recent progress using directed
RT-PCR cloning and DNA synthesis. The MGC now contains clones with the
entire protein-coding sequence for 92% of human and 89% of mouse genes
with curated RefSeq (NM-accession) transcripts, and for 97% of human and
96% of mouse genes with curated RefSeq transcripts that have one or more
PubMed publications, in addition to clones for more than 6300 rat genes.
These high-quality MGC clones and their sequences are accessible without
restriction to researchers worldwide.